SrasV1, Main, Exploration, bibRecord, 005E89

Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus.

Identifieur interne : 005E89 ( Main/Exploration ); précédent : 005E88; suivant : 005E90

Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus.

Auteurs : Boyd Yount [États-Unis] ; Kristopher M. Curtis ; Elizabeth A. Fritz ; Lisa E. Hensley ; Peter B. Jahrling ; Erik Prentice ; Mark R. Denison ; Thomas W. Geisbert ; Ralph S. Baric

Source :

Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2003.

RBID : pubmed:14569023

Descripteurs français

KwdFr :
- ADN complémentaire (génétique), Animaux, Cadres ouverts de lecture, Cellules Vero, Clonage moléculaire, Humains, Marqueurs génétiques, Syndrome respiratoire aigu sévère (virologie), Séquence consensus, Séquence nucléotidique, Techniques génétiques, Transcription génétique, Virus du SRAS (génétique), Virus du SRAS (pathogénicité).
MESH :
- génétique : ADN complémentaire, Virus du SRAS.
- pathogénicité : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Cadres ouverts de lecture, Cellules Vero, Clonage moléculaire, Humains, Marqueurs génétiques, Séquence consensus, Séquence nucléotidique, Techniques génétiques, Transcription génétique.

English descriptors

KwdEn :
- Animals, Base Sequence, Chlorocebus aethiops, Cloning, Molecular, Consensus Sequence, DNA, Complementary (genetics), Genetic Markers, Genetic Techniques, Humans, Open Reading Frames, SARS Virus (genetics), SARS Virus (pathogenicity), Severe Acute Respiratory Syndrome (virology), Transcription, Genetic, Vero Cells.
MESH :
- chemical , genetics : DNA, Complementary.
- genetics : SARS Virus.
- pathogenicity : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Base Sequence, Chlorocebus aethiops, Cloning, Molecular, Consensus Sequence, Genetic Markers, Genetic Techniques, Humans, Open Reading Frames, Transcription, Genetic, Vero Cells.

Abstract

A previously undescribed coronavirus (CoV) is the etiologic agent responsible for severe acute respiratory syndrome (SARS). Using a panel of contiguous cDNAs that span the entire genome, we have assembled a full-length cDNA of the SARS-CoV Urbani strain, and have rescued molecularly cloned SARS viruses (infectious clone SARS-CoV) that contained the expected marker mutations inserted into the component clones. Recombinant viruses replicated as efficiently as WT virus and both were inhibited by treatment with the cysteine proteinase inhibitor (2S,3S)-transepoxysuccinyl-L-leucylamido-3-methylbutane ethyl ester. In addition, subgenomic transcripts were initiated from the consensus sequence ACGAAC in both the WT and infectious clone SARS-CoV. Availability of a SARS-CoV full-length cDNA provides a template for manipulation of the viral genome, allowing for the rapid and rational development and testing of candidate vaccines and therapeutics against this important human pathogen.

DOI: 10.1073/pnas.1735582100
PubMed: 14569023

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus.</title>
<author><name sortKey="Yount, Boyd" sort="Yount, Boyd" uniqKey="Yount B" first="Boyd" last="Yount">Boyd Yount</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology and Microbiology, University of North Carolina, Chapel Hill, NC 27599-7435, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology and Microbiology, University of North Carolina, Chapel Hill, NC 27599-7435</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Curtis, Kristopher M" sort="Curtis, Kristopher M" uniqKey="Curtis K" first="Kristopher M" last="Curtis">Kristopher M. Curtis</name>
</author>
<author><name sortKey="Fritz, Elizabeth A" sort="Fritz, Elizabeth A" uniqKey="Fritz E" first="Elizabeth A" last="Fritz">Elizabeth A. Fritz</name>
</author>
<author><name sortKey="Hensley, Lisa E" sort="Hensley, Lisa E" uniqKey="Hensley L" first="Lisa E" last="Hensley">Lisa E. Hensley</name>
</author>
<author><name sortKey="Jahrling, Peter B" sort="Jahrling, Peter B" uniqKey="Jahrling P" first="Peter B" last="Jahrling">Peter B. Jahrling</name>
</author>
<author><name sortKey="Prentice, Erik" sort="Prentice, Erik" uniqKey="Prentice E" first="Erik" last="Prentice">Erik Prentice</name>
</author>
<author><name sortKey="Denison, Mark R" sort="Denison, Mark R" uniqKey="Denison M" first="Mark R" last="Denison">Mark R. Denison</name>
</author>
<author><name sortKey="Geisbert, Thomas W" sort="Geisbert, Thomas W" uniqKey="Geisbert T" first="Thomas W" last="Geisbert">Thomas W. Geisbert</name>
</author>
<author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2003">2003</date>
<idno type="RBID">pubmed:14569023</idno>
<idno type="pmid">14569023</idno>
<idno type="doi">10.1073/pnas.1735582100</idno>
<idno type="wicri:Area/PubMed/Corpus">003133</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003133</idno>
<idno type="wicri:Area/PubMed/Curation">003133</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003133</idno>
<idno type="wicri:Area/PubMed/Checkpoint">003139</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003139</idno>
<idno type="wicri:Area/Ncbi/Merge">000399</idno>
<idno type="wicri:Area/Ncbi/Curation">000399</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000399</idno>
<idno type="wicri:doubleKey">0027-8424:2003:Yount B:reverse:genetics:with</idno>
<idno type="wicri:Area/Main/Merge">006372</idno>
<idno type="wicri:Area/Main/Curation">005E89</idno>
<idno type="wicri:Area/Main/Exploration">005E89</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus.</title>
<author><name sortKey="Yount, Boyd" sort="Yount, Boyd" uniqKey="Yount B" first="Boyd" last="Yount">Boyd Yount</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology and Microbiology, University of North Carolina, Chapel Hill, NC 27599-7435, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology and Microbiology, University of North Carolina, Chapel Hill, NC 27599-7435</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Curtis, Kristopher M" sort="Curtis, Kristopher M" uniqKey="Curtis K" first="Kristopher M" last="Curtis">Kristopher M. Curtis</name>
</author>
<author><name sortKey="Fritz, Elizabeth A" sort="Fritz, Elizabeth A" uniqKey="Fritz E" first="Elizabeth A" last="Fritz">Elizabeth A. Fritz</name>
</author>
<author><name sortKey="Hensley, Lisa E" sort="Hensley, Lisa E" uniqKey="Hensley L" first="Lisa E" last="Hensley">Lisa E. Hensley</name>
</author>
<author><name sortKey="Jahrling, Peter B" sort="Jahrling, Peter B" uniqKey="Jahrling P" first="Peter B" last="Jahrling">Peter B. Jahrling</name>
</author>
<author><name sortKey="Prentice, Erik" sort="Prentice, Erik" uniqKey="Prentice E" first="Erik" last="Prentice">Erik Prentice</name>
</author>
<author><name sortKey="Denison, Mark R" sort="Denison, Mark R" uniqKey="Denison M" first="Mark R" last="Denison">Mark R. Denison</name>
</author>
<author><name sortKey="Geisbert, Thomas W" sort="Geisbert, Thomas W" uniqKey="Geisbert T" first="Thomas W" last="Geisbert">Thomas W. Geisbert</name>
</author>
<author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name>
</author>
</analytic>
<series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title>
<idno type="ISSN">0027-8424</idno>
<imprint><date when="2003" type="published">2003</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Base Sequence</term>
<term>Chlorocebus aethiops</term>
<term>Cloning, Molecular</term>
<term>Consensus Sequence</term>
<term>DNA, Complementary (genetics)</term>
<term>Genetic Markers</term>
<term>Genetic Techniques</term>
<term>Humans</term>
<term>Open Reading Frames</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (pathogenicity)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Transcription, Genetic</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term>
<term>Animaux</term>
<term>Cadres ouverts de lecture</term>
<term>Cellules Vero</term>
<term>Clonage moléculaire</term>
<term>Humains</term>
<term>Marqueurs génétiques</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Séquence consensus</term>
<term>Séquence nucléotidique</term>
<term>Techniques génétiques</term>
<term>Transcription génétique</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (pathogénicité)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Base Sequence</term>
<term>Chlorocebus aethiops</term>
<term>Cloning, Molecular</term>
<term>Consensus Sequence</term>
<term>Genetic Markers</term>
<term>Genetic Techniques</term>
<term>Humans</term>
<term>Open Reading Frames</term>
<term>Transcription, Genetic</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cadres ouverts de lecture</term>
<term>Cellules Vero</term>
<term>Clonage moléculaire</term>
<term>Humains</term>
<term>Marqueurs génétiques</term>
<term>Séquence consensus</term>
<term>Séquence nucléotidique</term>
<term>Techniques génétiques</term>
<term>Transcription génétique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A previously undescribed coronavirus (CoV) is the etiologic agent responsible for severe acute respiratory syndrome (SARS). Using a panel of contiguous cDNAs that span the entire genome, we have assembled a full-length cDNA of the SARS-CoV Urbani strain, and have rescued molecularly cloned SARS viruses (infectious clone SARS-CoV) that contained the expected marker mutations inserted into the component clones. Recombinant viruses replicated as efficiently as WT virus and both were inhibited by treatment with the cysteine proteinase inhibitor (2S,3S)-transepoxysuccinyl-L-leucylamido-3-methylbutane ethyl ester. In addition, subgenomic transcripts were initiated from the consensus sequence ACGAAC in both the WT and infectious clone SARS-CoV. Availability of a SARS-CoV full-length cDNA provides a template for manipulation of the viral genome, allowing for the rapid and rational development and testing of candidate vaccines and therapeutics against this important human pathogen.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Caroline du Nord</li>
</region>
</list>
<tree><noCountry><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name>
<name sortKey="Curtis, Kristopher M" sort="Curtis, Kristopher M" uniqKey="Curtis K" first="Kristopher M" last="Curtis">Kristopher M. Curtis</name>
<name sortKey="Denison, Mark R" sort="Denison, Mark R" uniqKey="Denison M" first="Mark R" last="Denison">Mark R. Denison</name>
<name sortKey="Fritz, Elizabeth A" sort="Fritz, Elizabeth A" uniqKey="Fritz E" first="Elizabeth A" last="Fritz">Elizabeth A. Fritz</name>
<name sortKey="Geisbert, Thomas W" sort="Geisbert, Thomas W" uniqKey="Geisbert T" first="Thomas W" last="Geisbert">Thomas W. Geisbert</name>
<name sortKey="Hensley, Lisa E" sort="Hensley, Lisa E" uniqKey="Hensley L" first="Lisa E" last="Hensley">Lisa E. Hensley</name>
<name sortKey="Jahrling, Peter B" sort="Jahrling, Peter B" uniqKey="Jahrling P" first="Peter B" last="Jahrling">Peter B. Jahrling</name>
<name sortKey="Prentice, Erik" sort="Prentice, Erik" uniqKey="Prentice E" first="Erik" last="Prentice">Erik Prentice</name>
</noCountry>
<country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Yount, Boyd" sort="Yount, Boyd" uniqKey="Yount B" first="Boyd" last="Yount">Boyd Yount</name>
</region>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005E89 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005E89 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:14569023
   |texte=   Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:14569023" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

Serveur d'exploration SRAS

Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus.

Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.